Interfacial layer between root dentin and mineral trioxide aggregate mixed with phosphate-buffered saline solution in a root-canal model: A preliminary study
Keywords:dentin, interfacial layer, mineral trioxide aggregate, phosphate-buffered saline, root canal
The objective of this preliminary study was to investigate the effect of mixing phosphate-buffered saline (PBS) solution with mineral trioxide aggregate (MTA) on interfacial-layer formation between MTA orthograde filling and root dentin in a root-canal model under scanning electron microscopy (SEM) and energy-dispersive x-ray spectroscopy (EDXS). Each of the four human mandibular premolars was horizontally sectioned to obtain a 12-mm length of the root. The root canal was sequentially prepared by a series of peeso-reamers (sizes 2−4) to simulate a root-canal specimen of the immature tooth with an opened apex. The two specimens were filled with MTA (ProRoot MTA) mixed with PBS solution (MTA/PBS) while the other two specimens were filled with MTA mixed with distilled water (MTA/DTW). The MTA was packed into the canal for an 8 mm length, and the 4-mm remaining canal was filled with small moist cotton and a temporary restorative material. All specimens were stored in PBS solution for 4 weeks. For the experimental specimens of MTA/PBS, the roots were sectioned at the coronal, middle, and apical levels of MTA whereas the roots of the controls (MTA/DTW) were sectioned at the middle level. The specimens were prepared for SEM and EDXS examination. The representative interfaces and EDXS analysis were investigated at 3,000x magnification under SEM. SEM images and EDXS graphs of the MTA/DTW specimens showed no phosphate-containing interfacial layer was formed. SEM images and EDXS graphs of the MTA/PBS specimens showed a formation of the interfacial layer containing the phosphate element at all levels of the root canal. At the coronal and middle levels, the peaks of phosphate in the interfacial layer were lower than those in the root dentin. At the apical level, the peak of phosphate was as high as that in the dentin. In conclusion, MTA mixing with PBS solution tends to induce an interfacial layer formed between the MTA material and the root dentin, particularly at the apical level.
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