Interfacial layer between root dentin and mineral trioxide aggregate mixed with phosphate-buffered saline solution in a root-canal model: A preliminary study
Keywords:
dentin, interfacial layer, mineral trioxide aggregate, phosphate-buffered saline, root canalAbstract
The objective of this preliminary study was to investigate the effect of mixing phosphate-buffered saline (PBS) solution with mineral trioxide aggregate (MTA) on interfacial-layer formation between MTA orthograde filling and root dentin in a root-canal model under scanning electron microscopy (SEM) and energy-dispersive x-ray spectroscopy (EDXS). Each of the four human mandibular premolars was horizontally sectioned to obtain a 12-mm length of the root. The root canal was sequentially prepared by a series of peeso-reamers (sizes 2−4) to simulate a root-canal specimen of the immature tooth with an opened apex. The two specimens were filled with MTA (ProRoot MTA) mixed with PBS solution (MTA/PBS) while the other two specimens were filled with MTA mixed with distilled water (MTA/DTW). The MTA was packed into the canal for an 8 mm length, and the 4-mm remaining canal was filled with small moist cotton and a temporary restorative material. All specimens were stored in PBS solution for 4 weeks. For the experimental specimens of MTA/PBS, the roots were sectioned at the coronal, middle, and apical levels of MTA whereas the roots of the controls (MTA/DTW) were sectioned at the middle level. The specimens were prepared for SEM and EDXS examination. The representative interfaces and EDXS analysis were investigated at 3,000x magnification under SEM. SEM images and EDXS graphs of the MTA/DTW specimens showed no phosphate-containing interfacial layer was formed. SEM images and EDXS graphs of the MTA/PBS specimens showed a formation of the interfacial layer containing the phosphate element at all levels of the root canal. At the coronal and middle levels, the peaks of phosphate in the interfacial layer were lower than those in the root dentin. At the apical level, the peak of phosphate was as high as that in the dentin. In conclusion, MTA mixing with PBS solution tends to induce an interfacial layer formed between the MTA material and the root dentin, particularly at the apical level.
References
Parirokh M, Torabinejad M. Mineral trioxide aggregate: a comprehensive literature review-- Part I: chemical, physical, and antibacterial properties. J Endod. 2010; 36: 16-27.
Parirokh M, Torabinejad M. Mineral trioxide aggregate: a comprehensive literature review-- Part III: Clinical applications, drawbacks, and mechanism of action. J Endod. 2010; 36: 400-413.
Camilleri J. Hydration mechanisms of mineral trioxide aggregate. Int Endod J. 2007; 40: 462-470.
Gandolfi MG, Iacono F, Agee K, Siboni F, Tay F, Pashley DH, et al. Setting time and expansion in different soaking media of experimental
accelerated calcium-silicate cements and ProRoot MTA. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2009; 108: e39-45.
Camilleri J. Characterization of hydration products of mineral trioxide aggregate. Int Endod J. 2008; 41: 408-417.
Gandolfi MG, Taddei P, Tinti A, Prati C. Apatiteforming ability (bioactivity) of ProRoot MTA.
Int Endod J. 2010; 43: 917-929. 7. Lertmalapong P, Jantarat J, Srisatjaluk RL, Komoltri C. Bacterial leakage and marginal adaptation of various bioceramics as apical plug in open apex model. J Investig Clin Dent. 2019; 10: e12371.
Torabinejad M, Parirokh M. Mineral trioxide aggregate: a comprehensive literature review--part II: leakage and biocompatibility
investigations. J Endod. 2010; 36: 190-202.
Storm B, Eichmiller FC, Tordik PA, Goodell GG. Setting expansion of gray and white mineral trioxide aggregate and Portland cement. J Endod. 2008; 34: 80-82.
Hawley M, Webb TD, Goodell GG. Effect of varying water-to-powder ratios on the setting expansion of white and gray mineral trioxide aggregate. J Endod. 2010; 36: 1377-1379.
Dreger LA, Felippe WT, Reyes-Carmona JF, Felippe GS, Bortoluzzi EA, Felippe MC. Mineral trioxide aggregate and Portland cement promote biomineralization in vivo. J Endod. 2012; 38: 324-329.
Reyes-Carmona JF, Felippe MS, Felippe WT. Biomineralization ability and interaction of mineral trioxide aggregate and white portland cement with dentin in a phosphate-containing fluid. J Endod. 2009; 35: 731-736.
Reyes-Carmona JF, Felippe MS, Felippe WT. A phosphate-buffered saline intracanal dressing improves the biomineralization ability of mineral trioxide aggregate apical plugs. J Endod. 2010; 36: 1648-1652.
Han L, Okiji T. Bioactivity evaluation of three calcium silicate-based endodontic materials. Int Endod J. 2013; 46: 808-814.
Kim JR, Nosrat A, Fouad AF. Interfacial characteristics of Biodentine and MTA with dentine in simulated body fluid. J Dent. 2015; 43: 241-247.
Sarkar NK, Caicedo R, Ritwik P, Moiseyeva R, Kawashima I. Physicochemical basis of the biologic properties of mineral trioxide aggregate. J Endod. 2005; 31: 97-100.
Bozeman TB, Lemon RR, Eleazer PD. Elemental analysis of crystal precipitate from gray and white MTA. J Endod. 2006; 32: 425-428.
Bird DC, Komabayashi T, Guo L, Opperman LA, Spears R. In vitro evaluation of dentinal tubule penetration and biomineralization ability of a new root-end filling material. J Endod. 2012; 38: 1093-1096.
Tay FR, Pashley DH, Rueggeberg FA, Loushine RJ, Weller RN. Calcium phosphate phase transformation produced by the interaction of the portland cement component of white mineral trioxide aggregate with a phosphatecontaining fluid. J Endod. 2007; 33: 1347-1351.
Han L, Okiji T, Okawa S. Morphological and chemical analysis of different precipitates on mineral trioxide aggregate immersed in different fluids. Dent Mater J. 2010; 29: 512-517.
Allo BA, Rizkalla AS, Mequanint K. Hydroxyapatite formation on sol-gel derived poly(ε-caprolactone)/ bioactive glass hybrid biomaterials. Appl Mater Interfaces. 2012; 4: 3148-3156.
Żuk-Grajewska E, Saunders WP, Chadwick RG. Fracture resistance of human roots filled with mineral trioxide aggregate mixed with phosphate-buffered saline, with and without calcium hydroxide pre-medication. Int Endod J. 2021; 54: 439-453.
Parirokh M, Asgary S, Eghbal MJ, Ghoddusi J, Brink F, Askarifar S, et al. The Long-Term Effect of Saline and Phosphate Buffer Solution on MTA: An SEM and EPMA Investigation. Iran Endod J. 2007; 2: 81-86.
Reyes-Carmona JF, Felippe MS, Felippe WT. The biomineralization ability of mineral trioxide aggregate and Portland cement on dentin enhances the push-out strength. J Endod. 2010; 36: 286-291.
Zapf AM, Chedella SC, Berzins DW. Effect of additives on mineral trioxide aggregate setting reaction product formation. J Endod.
; 41: 88-91.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Thai Endodontic Journal
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Thai Endod Journal is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) license, unless otherwise stated. Please read our Policies in Copyright for more information.
