รีเจเนอเรทีฟ เอ็นโดดอนติกส์: รูปแบบ Cell-free และ Cell-based
Keywords:
Stem cells, Regenerative Endodontic Procedures, Pulp/dentin regenerationAbstract
Regenerative Endodontic Procedures (REPs) is one of the treatment options for immature permanent teeth with necrotic pulp. These procedures use the principle of tissue engineering in order to regenerate pulp/dentine complex. Stem cell recruited into the root canal is one of the crucial factors that affect tissue regeneration in REPs. Therefore, the ultimate goal of this treatment is not only the healing of apical periodontitis but also the regeneration of pulp/dentine complex. However, pulp/dentine regeneration in REPs teeth can not be achieved in histological evaluation. This literature review aims to review the literatures related to stem cells used for pulp/dentine regeneration from past to present and the direction of further researches.
References
Lin LM, Huang GT, Sigurdsson A, Kahler B. Clinical cell-based versus cell-free regenerative endodontics: clarification of concept and term. Int Endod J. 2021; 54: 887-901.
Cui D, Yu S, Zhou X, Liu Y, Gan L, Pan Y, et al. Roles of Dental Mesenchymal Stem Cells in the Management of Immature Necrotic Permanent Teeth. Front Cell Dev Biol. 2021; 9: 666186.
Galler KM, Eidt A, Schmalz G. Cell-free approaches for dental pulp tissue engineering. J Endod. 2014; 40: S41-5.
Lovelace TW, Henry MA, Hargreaves KM, Diogenes A. Evaluation of the delivery of mesenchymal stem cells into the root canal space of necrotic immature teeth after clinical regenerative endodontic procedure. J Endod. 2011; 37: 133-8.
Chrepa V, Henry MA, Daniel BJ, Diogenes A. Delivery of Apical Mesenchymal Stem Cells into Root Canals of Mature Teeth. J Dent Res.
; 94: 1653-9.
Galler KM, Buchalla W, Hiller KA, Federlin M, Eidt A, Schiefersteiner M, et al. Influence of root canal disinfectants on growth factor release from dentin. J Endod. 2015; 41: 363-8.
Civinini R, Macera A, Nistri L, Redl B, Innocenti M. The use of autologous blood-derived growth factors in bone regeneration. Clin Cases Miner Bone Metab. 2011; 8: 25-31.
Ruangsawasdi N, Zehnder M, Weber FE. Fibrin gel improves tissue ingrowth and cell differentiation in human immature premolars
implanted in rats. J Endod. 2014; 40: 246-50.
Becerra P, Ricucci D, Loghin S, Gibbs JL, Lin LM. Histologic study of a human immature permanent premolar with chronic apical abscess after revascularization/revitalization. J Endod. 2014; 40: 133-9.
Lei L, Chen Y, Zhou R, Huang X, Cai Z. Histologic and Immunohistochemical Findings of a Human Immature Permanent Tooth with Apical Periodontitis after Regenerative Endodontic Treatment. J Endod. 2015; 41: 1172-9.
Shimizu E, Jong G, Partridge N, Rosenberg PA, Lin LM. Histologic observation of a human immature permanent tooth with irreversible pulpitis after revascularization/regeneration procedure. J Endod. 2012; 38: 1293-7.
Torabinejad M, Faras H. A clinical and histological report of a tooth with an open apex treated with regenerative endodontics
using platelet-rich plasma. J Endod. 2012; 38: 864-8.
Martin G, Ricucci D, Gibbs JL, Lin LM. Histological findings of revascularized/revitalized immature permanent molar with apical periodontitis using platelet-rich plasma. J Endod. 2013; 39: 138-44.
Nosrat A, Kolahdouzan A, Hosseini F, Mehrizi EA, Verma P, Torabinejad M. Histologic Outcomes of Uninfected Human Immature Teeth Treated with Regenerative Endodontics: 2 Case Reports. J Endod. 2015; 41: 1725-9.
Shetty H, Shetty S, Kakade A, Desai R, Zhang CF, Neelakantan P. Cone-beam computed tomographic and histological investigation of
regenerative endodontic procedure in an immature mandibular second premolar with chronic apical abscess. J Investig Clin Dent.
; 9: e12352.
Austah O, Joon R, Fath WM, Chrepa V, Diogenes A, Ezeldeen M, et al. Comprehensive Characterization of 2 Immature Teeth Treated
with Regenerative Endodontic Procedures. J Endod. 2018; 44: 1802-11.
Huang GT, Liu J, Zhu X, Yu Z, Li D, Chen CA, et al. Pulp/Dentin Regeneration: It Should Be Complicated. J Endod. 2020; 46: S128-34.
Kim SG. Infection and Pulp Regeneration. Dent J (Basel). 2016; 4: 4.
Vishwanat L, Duong R, Takimoto K, Phillips L, Espitia CO, Diogenes A, et al. Effect of Bacterial Biofilm on the Osteogenic Differentiation of Stem Cells of Apical Papilla. J Endod. 2017; 43: 916-22.
Verma P, Nosrat A, Kim JR, Price JB, Wang P, Bair E, et al. Effect of Residual Bacteria on the Outcome of Pulp Regeneration In Vivo.
J Dent Res. 2017; 96: 100-6.
Almutairi W, Yassen GH, Aminoshariae A, Williams KA, Mickel A. Regenerative Endodontics: A Systematic Analysis of the Failed Cases.
J Endod. 2019; 45: 567-77.
Lee C, Song M. Failure of Regenerative Endodontic Procedures: Case Analysis and Subsequent Treatment Options. J Endod.
; 48: 1137-45.
Diogenes A, Hargreaves KM. Microbial Modulation of Stem Cells and Future Directions in Regenerative Endodontics.
J Endod. 2017; 43: s95-101.
Martin DE, De Almeida JF, Henry MA, Khaing ZZ, Schmidt CE, Teixeira FB, et al. Concentration-dependent effect of sodium
hypochlorite on stem cells of apical papilla survival and differentiation. J Endod. 2014; 40: 51-5.
Claudino Ribeiro JR, da Silveira Bueno CE, Bruno KF, Dos Reis S, de Martin AS, Fontana CE, et al. Impact of Sodium Hypochlorite on Organic Tissue Dissolution in the Periapical Region of Immature Permanent Teeth: An Ex Vivo Study. J Endod. 2022; 48: 555-60.
Trevino EG, Patwardhan AN, Henry MA, Perry G, Dybdal-Hargreaves N, Hargreaves KM, et al. Effect of irrigants on the survival of human stem cells of the apical papilla in a platelet-rich plasma scaffold in human root tips. J Endod. 2011; 37: 1109-15.
Althumairy RI, Teixeira FB, Diogenes A. Effect of dentin conditioning with intracanal medicaments on survival of stem cells of
apical papilla. J Endod. 2014; 40: 521-5.
Ruparel NB, Teixeira FB, Ferraz CC, Diogenes A. Direct effect of intracanal medicaments on survival of stem cells of the apical papilla.
J Endod. 2012; 38: 1372-5.
American Association of Endodontics. AAE Clinical considerations for a regenerative procedure revised 5/18/2021. 2021 [cited 2022
Feb 26]. Available from: https://www.aae.org/ specialty/wp-content/uploads/sites/2/2021/ 08/ClinicalConsiderationsApprovedByREC
pdf.
Liu C, Xiong H, Chen K, Huang Y, Huang Y, Yin X. Long-term exposure to pro-inflammatory cytokines inhibits the osteogenic/dentinogenic differentiation of stem cells from the apical papilla. Int Endod J. 2016; 49: 950-9.
Lertchirakarn V, Aguilar P. Effects of Lipopolysaccharide on the Proliferation and Osteogenic Differentiation of Stem Cells from
the Apical Papilla. J Endod. 2017; 43: 1835-40.
Tobias Duarte PC, Gomes-Filho JE, Ervolino E, Marçal Mazza Sundefeld ML, Tadahirowayama M, Lodi CS, et al. Histopathological condition of the remaining tissues after endodontic infection of rat immature teeth. J Endod. 2014; 40: 538-42.
Liao J, Al Shahrani M, Al-Habib M, Tanaka T, Huang GT. Cells isolated from inflamed periapical tissue express mesenchymal stem cell markers and are highly osteogenic. J Endod. 2011; 37: 1217-24.
Chrepa V, Pitcher B, Henry MA, Diogenes A. Survival of the Apical Papilla and Its Resident Stem Cells in a Case of Advanced Pulpal
Necrosis and Apical Periodontitis. J Endod. 2017; 43: 561-7.
Nada OA, El Backly RM. Stem Cells From the Apical Papilla (SCAP) as a Tool for Endogenous Tissue Regeneration. Front Bioeng Biotechnol. 2018; 6: 103.
Huang GT-J, Al-Habib M, Gauthier P. Challenges of stem cell-based pulp and dentin regeneration: a clinical perspective. Endodontic Topics. 2013; 28: 51-60.
Kim SG. A Cell-Based Approach to Dental Pulp Regeneration Using Mesenchymal Stem Cells: A Scoping Review. Int J Mol Sci. 2021; 22.
Nakashima M, Iohara K, Bottino MC, Fouad AF, Nör JE, Huang GT. Animal Models for Stem Cell-Based Pulp Regeneration: Foundation for Human Clinical Applications. Tissue Eng Part B Rev. 2019; 25: 100-13.
Huang GT, Yamaza T, Shea LD, Djouad F, Kuhn NZ, Tuan RS, et al. Stem/progenitor cell-mediated de novo regeneration of dental
pulp with newly deposited continuous layer of dentin in an in vivo model. Tissue Eng Part A. 2010; 16: 605-15.
Li X, Ma C, Xie X, Sun H, Liu X. Pulp regeneration in a full-length human tooth root using a hierarchical nanofibrous microsphere system. Acta Biomater. 2016; 35: 57-67.
Iohara K, Imabayashi K, Ishizaka R, Watanabe A, Nabekura J, Ito M, et al. Complete pulp r e g e n e r a t i o n a f t e r p u l p e c t o m y by transplantation of CD105+ stem cells with stromal cell-derived factor-1. Tissue Eng Part A. 2011; 17: 1911-20.
Nakashima M, Iohara K. Pulp Regeneration by Harnessing Dental Pulp Stem Cells. In: Hideharu H, Minoru U, editors. New Trends in Tissue Engineering and Regenerative Medicine. Rijeka: IntechOpen; 2014. p. Ch. 3.
Iohara K, Murakami M, Takeuchi N, Osako Y, Ito M, Ishizaka R, et al. A novel combinatorial therapy with pulp stem cells and granulocyte colony-stimulating factor for total pulp regeneration. Stem Cells Transl Med. 2013; 2: 521-33.
Murakami M, Hayashi Y, Iohara K, Osako Y, Hirose Y, Nakashima M. Trophic Effects and Regenerative Potential of Mobilized
Mesenchymal Stem Cells From Bone Marrow and Adipose Tissue as Alternative Cell Sources for Pulp/Dentin Regeneration. Cell Transplant. 2015; 24: 1753-65.
Ishizaka R, Iohara K, Murakami M, Fukuta O, Nakashima M. Regeneration of dental pulp following pulpectomy by fractionated stem/
progenitor cells from bone marrow and adipose tissue. Biomaterials. 2012; 33: 2109-18.
Iohara K, Murakami M, Nakata K, Nakashima M. Age-dependent decline in dental pulp regeneration after pulpectomy in dogs.
Exp Gerontol. 2014; 52: 39-45.
Iohara K, Utsunomiya S, Kohara S, Nakashima M. Allogeneic transplantation of mobilized dental pulp stem cells with the mismatched dog leukocyte antigen type is safe and efficacious for total pulp regeneration. Stem Cell Res Ther. 2018; 9: 116.
Zhu X, Liu J, Yu Z, Chen CA, Aksel H, Azim AA, et al. A Miniature Swine Model for Stem CellBased De Novo Regeneration of Dental Pulp and Dentin-Like Tissue. Tissue Eng Part C Methods. 2018; 24: 108-20.
El Ashiry EA, Alamoudi NM, El Ashiry MK, Bastawy HA, El Derwi DA, Atta HM. Tissue Engineering of Necrotic Dental Pulp of
Immature Teeth with Apical Periodontitis in Dogs: Radiographic and Histological Evaluation. J Clin Pediatr Dent. 2018; 42: 373-82.
Nakashima M, Iohara K, Murakami M, Nakamura H, Sato Y, Ariji Y, et al. Pulp regeneration by transplantation of dental pulp stem cells in pulpitis: a pilot clinical study. Stem Cell Res Ther. 2017; 8: 61.
Xuan K, Li B, Guo H, Sun W, Kou X, He X, et al. Deciduous autologous tooth stem cells regenerate dental pulp after implantation into
injured teeth. Sci Transl Med. 2018; 10.
Brizuela C, Meza G, Urrejola D, Quezada MA, Concha G, Ramírez V, et al. Cell-Based Regenerative Endodontics for Treatment of Periapical Lesions: A Randomized, Controlled Phase I/II Clinical Trial. J Dent Res. 2020; 99: 523-9.
Meza G, Urrejola D, Saint Jean N, Inostroza C, López V, Khoury M, et al. Personalized Cell Therapy for Pulpitis Using Autologous Dental Pulp Stem Cells and Leukocyte Platelet-rich Fibrin: A Case Report. J Endod. 2019; 45: 144-9.
Nakashima M, Fukuyama F, Iohara K. Pulp Regenerative Cell Therapy for Mature Molars: A Report of 2 Cases. J Endod. 2022; 48: 1334-40.
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